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It is a set of investigations
aimed to unmask any small abnormalities leading
to coronary artery diseases. 12hrs fasting is
required. It also includes Chest X-Ray and ECG
along with the lab tests (CBC, ESR, glucose, uric
acid, cholesterol, HDL cholesterol, triglycerides
& Apolipoproteins). The profile will be complete
if combined with Treadmill Test and Echocardiography. |
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This profile is done
to detect liver and biliary tract disorders. It
includes U/S of the liver along with the lab tests
(total proteins, albumin, bilirubin direct & total,
alkaline phosphatase, SGOT, SGPT, GGTP and HBsAg). |
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It is a set of investigations
aimed at accurate diagnosis of connective tissue
disorders. It includes Haemogram, CRP, RA, ANA,
LE cell, serum proteins and uric acid. |
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It is a set of investigations
aimed at early detection of complication of hypertension.
12 hrs fasting is necessary. It Includes Chest
X- ray, ECG along with the lab tests (CBC, ESR,
urine routine or 24 hrs urine protein, glucose,
urea, uric acid, creatinine, cholesterol, HDL
cholesterol, triglycerides, sodium, potassium
and chlorides.) |
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It is a set of investigations
aimed at early diagnosis of kidney & Urinary tract
disorders. Fasting is necessary. It also includes
U/S of Kidney & Bladder along with the lab tests
(CBC, ESR, 24 hrs urine protein, creatinine clearance,
BUN, creatinine, uric acid, proteins, albumin,
calcium, phosphorous, sodium, potassium, chlorides
& alkaline phosphatase). |
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It is a set of investigations
aimed to detect the abnormalities of lipid levels,
which can ultimately lead to heart disease & stroke.
The test includes LDL, HDL, VLDL, total cholesterol
& triglycerides. |
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It is a set of investigations
aimed to screen the mother for transmissible diseases
to the child. The package includes VDRL, HIV,
HBsAg, CBC, FPG/RPG, urea, creatinine, blood group
& Rh type and bleeding & clotting time. |
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It is total body
profile with tests for screening all the sytems
of body for common ailments. |
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It is a set of investigations
aimed to diagnose pancreatitis and associated
metabolic abnormalities. Tests include triglycerides,
amylase, calcium, lipase and glucose. |
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Set of investigations
for the accurate diagnosis of Anemia and Jaundice
in children. Tests include haemogram, G6PD assay
, foetal haemoglobin, reticulocyte count, FDP
and osmotic fragility. |
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For diagnosis & treatment
of joint pains. Test includes ESR, CBC, CRP, Antinuclear
antibody, Uric acid & Rheumatoid factor (RA). |
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It is a set of investigations
aimed to detect the cause of infertility in both
male & female patients. Test includes FSH, LH,
Prolactin, Progesterone and Testosterone. |
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LFT is most often
employed to determine
1 The presence of liver disease
2 Type of liver disease
3 The extent and progression/regression of liver
disease. Tests included under this package are
- Bilirubin total & direct, Alkaline Phosphatase,
Total Protein, Albumin, Globulins, SGOT, SGPT
& GGTP |
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It
is a primary screening test for kidney disorders
& to monitor the patient who is already suffering
from the disorder. Test includes Urea, Creatinine,
CBC & Urine Complete. |
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PROFILES |
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| CORONARY
PROFILE |
| HEPATIC
PROFILE |
| COLLAGEN
DISORDER PROFILE |
| HYPERTENSION
PROFILE |
| RENAL
PROFILE |
| LIPID
PROFILE |
| PRENATAL
PROFILE |
| PAN
ORGAN PROFILE |
| PANCREATIC
PROFILE |
| HAEMOLYTIC
PROFILE |
| ARTHRITIS
PACKAGE |
| FERTILITY
PROFILE |
| LIVER
FUNCTION TEST (LFT) |
| KIDNEY
FUNCTION TEST |
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These are screening
tests for diabetes mellitus. |
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Fasting plasma glucose
more than 127 mg/dL on more than one occasion
and postprandial plasma glucose more than 200
mg/dL, after a mixed meal or following oral load
of 75g glucose strongly suggests the diagnosis
of diabetes mellitus. Low fasting blood glucose
is associated with hypothyroidism, hypoadrenalism,
hypopitutarism, insulinomas, excess dosage of
insulin/tablets, excess alcohol intake, massive
liver disease and glycogen storage disorders.
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Glucose Tolerance
test determines the ability of an individual to
utilize a given quantity of glucose given orally.
This test is used to diagnose diabetes mellitus
in those individuals whose plasma glucose levels
are either normal or marginally elevated. In suspected
diabetes the patient displays abnormal GTT. The
test is usually carried out in the morning after
a night fast. Interpretation of test results is
done according to a set of criteria. |
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This is a test requested
to assess the control of glucose levels in treated
diabetic patients. The test result indicates average
glucose control over past 2 months. This test
is also used by diabetologists to assess the compliance
of a patient to a given treatment plan. |
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Determination of
serum cholesterol is requested to predict/modify
the risk of coronary artery disease and stroke.
Increased total cholesterol levels are usually
due to increase in LDL cholesterol which is the
bad cholesterol and is found in hypothyroidism,
diabetes mellitus, liver diseases, nephrotic syndrome
and pregnancy. |
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Increased
LDL & VLDL cholesterol is noted with increasing
age and obesity. Low concentration of HDL cholesterol
is associated with |
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higher risk of coronary
heart disease even in the absence of other risk
factors. HDL in combination with total cholesterol
is a good index of the risk of coronary heart
disease. |
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Elevated triglyceride
levels are associated with increased LDL cholesterol
and decreased HDL cholesterol. Increased triglyceride
levels are found in diabetes mellitus, chronic
hepatitis, alcoholism, obesity and hypothyroidism.
Increased triglyceride levels lead to heart diseases
& stroke. |
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Increase in serum
urea may occur in number of diseases in addition
to those in which kidneys are primarily involved.
Reduced blood flow to kidneys, shock, blood loss,
dehydration, crush injuries, acute renal failure,
stones in urinary tract, tumor, inflammation,
diet rich in proteins etc. are some of the causes.
Decreased level of urea nitrogen is associated
with liver failure, over hydration, malnutrition
etc. |
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This test gives an
indication of the degree of renal impairment.
In cases of severe renal failure, creatinine may
increase from normal 0.6 - 1.4 mg to 10,20,30
mg per 100 ml. Serum and 24 hrs urinary creatinine
are estimated simultaneously to determine creatinine
clearance, which is an indicator of filtration
capacity of kidneys |
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Bilirubin if accumulated
in excess causes jaundice. This test is performed
as a part of liver function test and also requested
individually as screening test for liver disease.
This test is requested to know the cause of jaundice
& follow up. |
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Albumin is a protein
that occurs in serum portion of blood. The changing
levels of serum albumin provide valuable indices
of severity & progress in hepatic disease. Decreased
levels of albumin may be found in certain conditions
such as cirrhosis of the liver, other liver disorders
causing extensive damage to the organ, malnutrition
and nephrotic syndrome. |
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Uric Acid is an important
marker used in the diagnosis of gout. It is also
increased in acute stages of infectious diseases,
severe uremia, toxemia of pregnancy and leukemia.
24 hrs urinary excretion of uric acid is requested
in patients with renal stones made-up of uric
acid. |
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Increase
in both transferases is a common finding in liver
diseases in which liver cells have been damaged.
These tests are useful in assessing the severity
and monitoring the disease. SGOT levels are increased
following |
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heart
attack, diseases involving skeletal muscles, pulmonary
embolism and acute pancreatitis. In newborn children
these test are useful in classifying the cause
of jaundice |
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An elevated serum
GGTP level is indicative of disease of the liver,
biliary tract and pancreas. GGTP is particularly
helpful in clinical assessment of alcoholic cirrhosis.
GGTP is used |
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as a valuable diagnostic aid in differentiating
bone and liver disease in conjunction with alkaline
phosphatase. |
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Request for the analysis
of serum acid phosphatase is often done in male
patients with suspected prostatic cancer. Acid
phosphatase is |
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also
present in very high concentrations in semen a |
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fact utilised in
forensic medicine in the investigations of rape
offences. |
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Elevation of serum
alkaline phosphatase indicates obstructive disease
of liver and biliary system (e.g. obstructive
jaundice), diseases of bone such as rickets, hyperparathyroidism,
Paget's disease of bone, bone cancer etc. The
determination of tissue of origin gives more information
about the underlying pathologic process. |
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LDH is commonly requested
enzyme marker that indicates injury to the cells.
Serum LDH activity is elevated in diseases of
heart involving the muscular layer, liver diseases,
haemolysis, malignant diseases and disease of
skeletal muscles (Progressive muscular dystrophy).
The diagnostic efficiency of LDH is increased
further when the fractions of LDH, which originate
from different tissues, are estimated individually. |
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Request
for serum CPK level is made in case of suspected
cases of heart attack and muscle disease. Determination
of serum CPK-MB activity |
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is
a sensitive indicator of damage to muscular layer
of heart & thus plays a major role in the
differential diagnosis of |
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chest
pain and monitoring the response to treatment
in myocardial infarction. |
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Serum Iron is reduced
in iron deficiency anaemia, in anaemia of chronic
disorders and infections and in malignancies such
as Hodgkin's disease. Measurement of serum Iron
should always be combined with TIBC. TIBC-Total
Iron Binding Capacity measures the transferrin
concentration & its iron binding capabilities.
TIBC is raised in nutritional iron deficiency
and iron deficiency due to chronic blood loss,
oral contraceptive use etc. Decreased levels of
TIBC are caused by cancer, liver disease, rheumatoid
arthritis etc. |
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It is an enzyme that
is found in plasma which is required to inactivate
a short acting anesthetic drug succinyl choline.
This enzyme is deficient/inactive in a few ethnic
groups, which will prolong the apnoea (apnoea:loss
of breathing) when the drug is administered. This
test is used as a screening procedure to assess
the sensitivity of a person to succinyl choline.
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A request for serum
calcium is made in patients with parathyroid diseases,
Vitamin D imbalance, chronic renal failure & cancerous
conditions. Low calcium value appears more frequently
in osteomalacia/rickets. In case of renal failure
the calcium level falls. Increased calcium levels
are found in hyperparathyroidism and vitamin D
intoxication. In neonates decreased calcium level
is one of the important causes of convulsions.
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Serum
electrolytes is an important parameter requested
in any condition leading to fluid and electrolyte
imbalance. Such imbalances are found |
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in
diseases of kidney, adrenal glands, digestive
system giving rise to massive fluid loss, severe
burns, and |
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diabetes
insipidus. Serum electrolyte results plays an
important role in management of critically ill
patients. |
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A significant increase
of the serum inorganic phosphorus level is seen
in the case of rickets associated with renal failure.
Other conditions causing raised inorganic phosphate
levels are respiratory acidosis, lactic acidosis
and vitamin D intoxication can be associated with
hypoparathyroidism. A decrease of serum inorganic
phosphate could is seen in hyperparathyroidism,
hypercalcemia (increase in serum calcium level)
due to any cause and respiratory alkalosis. |
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This test is performed
for the diagnosis of diseases of pancreas that
lead to damage of pancreatic cells ex. pancreatitis,
and for the investigation of pancreas function
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Lipase is an enzyme
released from damaged pancreas. This test helps
in the diagnosis of acute pancreatitis along with
serum amylase level. Elevation in patients with
mumps strongly suggests significant pancreatic
as well as salivary gland involvement by the disease. |
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This is an important
test requested to assess the ability of kidneys
to filter the blood, otherwise known as glomerular
filtration rate. This test is used as a tool by
clinicians to recommend dialysis/kidney transplantation
to a patient in renal failure. |
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Chemical analysis
of a stone removed from the body gives information
about the probable cause that led to stone formation
and necessary precautions to be taken to prevent
further recurrence. |
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CLINICAL
CHEMISTRY |
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 |
Glucose
Fasting /Random, FBS/PPBS & Glucose Fast & Post Glucose
(75g) |
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|
Glucose Tolerance Test |
| Glycosylated
Hemoglobin |
| Cholesterol
Total, HDL, LDL & VLDL Cholesterol |
| Triglycerides |
| Urea/Urea
Nitrogen |
| Creatinine |
| Bilirubin
Total & Direct |
| Albumin |
| Uric
Acid |
| Aspartate
Amino Transferase (SGOT) & Alanine Amino Transferase (SGPT) |
| Gamma
Glutamyl Transferase (GGT or GGTP) |
| Acid
Phosphatase Total & Acid Phosphatase Prostatic Fraction |
| Alkaline
Phosphatase |
| Lactic
Dehydrogenase (LDH) |
| Creatinine
Phosphokinase Total (CPK) & CPK-MB |
| Iron
& TIBC |
| Pseudo
Cholinesterase |
| Calcium |
| Serum
Electrolytes [Sodium, Potassium & Chloride] |
| Inorganic
Phosphorous |
| Amylase
|
| Lipase |
| Creatinine
Clearance Test |
| Stone
Analysis (Gall/renal) |
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The laboratory
testing of endocrine function and the measurement
of specific hormones assist in establishing a
definite diagnosis. The rapid |
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development
of radioimmunoassay & competitive protein
binding techniques is making possible more precise
identification of clinical endocrine |
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abnormalities.
Tumour markers are included under this class because
the method used to measure them is same as that
of hormones. |
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It is a screening
test for thyroid gland abnormalities. T4 and to
some extent T3 are the hormones secreted by thyroid
gland, which is regulated by a pituitary hormone
TSH. Thyroid hormones affect the rate of metabolism,
growth & development. Excess thyroid hormones
cause thyrotoxicosis with clinical evidence of
autonomic nervous system over activity and hyper
metabolism. Deficiency of thyroid hormones cause
failure of growth and development and mental retardation
in infants and children which is known as cretinism,
whereas in adults it leads to hypothyroidism,
a constellation symptoms characterized by weight
gain, skin changes, lethargy, intolerance to cold
and menstrual irregularities. |
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T3 is derived from
T4. Elevation of T3 occurs in a condition called
T3 toxicosis, which is one of the causes of hyperthyroidism.
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T4 is the primary
thyroid hormone secreted by thyroid gland. T4
level is increased or decreased in primary thyroid
disease. Non-specific elevations occur in a group
of diseases unrelated to thyroid gland, which
are termed as nonthyroidal illnesses. In such
cases free T4, that is the fraction of T4 in blood,
which is not bound to thyroid binding proteins,
remains within normal limits. T4 level is also
used to monitor the response to treatment in patients
with primary thyroid disease. |
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TSH is
a hormone secreted by pituitary gland that stimulates
the thyroid gland to synthesize and secrete T4
& T3. TSH level is elevated in primary |
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hypothyroidism
and depressed in primary hyperthyroidism. TSH
alone is used as screening test to detect any
thyroid abnormalities. TSH and free T4 together
are |
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sufficient to diagnose/rule
out most of the primary thyroid disorders. |
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These
two hormones, collectively known as pituitary
gonadotrophins, are secreted by pituitary gland
and acts on testes in males and ovaries in |
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females
to maintain normal reproductive functions.
These hormones |
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estimated in blood
to determine whether a gonadal insufficiency is
primary or due to deficient stimulation by pituitary
hormones. |
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Estradiol is a hormone
secreted by developing ovarian follicles under
the influence of pituitary gonadotrophins. Estrone
and estriol are breakdown products of estradiol.
Measurement of individual estrogen or total estrogens
has limited clinical utility Estrone measurement
is indicated in female patients presenting with
postmenopausal bleeding. Estriol is the major
estrogen secreted by placenta and hence used as
a marker of placental dysfunction. Estradiol level
tends to fall as women reach the age of menopause.
Estradiol measurement is indicated in male patients
with gynecomastia (development of breast in males)
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Testosterone is the
male sex hormone secreted by testes under the
influence of pituitary gonadotrophins. Testosterone
along with pituitary gonadotrophins is useful
in the assessment and classification of hypogonadism
(improper functioning of testes) in males. These
measurements are also useful in the detection
of ovarian & adrenal tumors in woman that lead
to development of male sexual characteristics. |
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Prolactin is a hormone
secreted by pituitary gland. This investigation
is helpful in the diagnosis, management & follow-up
of a prolactin-secreting tumor in women who have
secondary amenorrhea (absence or suppression of
menses) and/or galactorrhea (secretion of milk
from the breast) with hyperprolactinemia. In males
prolactin measurement is indicated in cases of
gynecomastia and infertility. It is also useful
in differentiating major form of epileptic fits
from those of minor varieties |
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HGH is secreted by
pituitary gland. Its main function is to stimulate
growth of all nonendocrine tissues in the body.
Deficiency of HGH causes dwarfism & its excess
causes gigantism in children and acromegaly in
adults. |
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Parathyroid hormone
is produced by parathyroid gland and is one of
the major factors involved in the regulation of
calcium concentration in extracellular fluid.
Investigation is of importance in distinguishing
nonparathyroid from parathyroid causes of hypercalcemia
(increased calcium level in the blood). Increased
PTH values are seen in primary hyperparathyroidism,
chronic renal failure, pseudohyperparathyroidism,
vitamin D deficiency, nutritional calcium deficiency
etc. Decreased PTH values occur in primary hypoparathyroidism,
sarcoidosis, milk alkali syndrome, vitamin D intoxication
etc. |
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Cortisol is a hormone
secreted from adrenal gland. This is a test of
adrenal gland function. Decreased levels are seen
in Addison's disease, Hypothyroidism etc. Increased
levels are found in stress, Cushing's syndrome
etc. Single random measurement of cortisol level
does not give much diagnostic information. This
test will be useful only when samples are drawn
from the patient at specified time in a day or
conducted as a part of adrenal gland stimulation
test. |
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These
substances are breakdown products of steroids.
Their estimation is indicated in the |
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screening
for endocrine disturbances of the adrenal glands
& testes. |
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This investigation
is usually needed for a person with hypertension
suspected to be having a tumor of chromaffin cells
of the adrenal medulla. The test is requested
most in young people with hypertension. |
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This stimulates production
of all adrenocortical hormones. Its deficiency
causes pituitary adrenal insufficiency; its excess
causes Cushing's disease. |
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A fetal blood protein
produced in adults by cancer cells & normally
in the amniotic fluid of pregnant women, with
high or low levels tending to be associated with
certain birth defects. Serum AFP level is used
as a tumour marker in primary liver cancer and
teratoblastoma of ovary and testes. |
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CEA is a protein
present in fetal gut tissues during the first
two trimesters of pregnancy. Cancer cells in adults
produce CEA. CEA is used as tumour marker in cancer
of large intestine and as supplementary tumour
marker in cancers of various organs such as cervix,
stomach, pancreas, ovary, prostate and liver.
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PSA is produced by
prostate gland. Varying increase in PSA level
is observed in disease of prostate gland with
maximum increase in cancer of prostate. PSA is
used as tumour marker to diagnose and to monitor
the response to treatment in patients with prostatic
cancer. |
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ENDOCRINOLOGY/TUMOUR
MARKERS |
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Thyroid
Screen (T3, T4 & TSH) |
 |
| Triodothyronine
(T3) |
| Thyroxine
(T4) |
| Thyroid
Stimulating Hormone (TSH) |
| Follicle
Stimulating Hormone (FSH) & Leutinizing Hormone (LH) |
| Estradiol,
Estrone and Estriol |
| Testosterone |
| Prolactin |
| Human
Growth Hormone (HGH) |
| Parathyroid
Hormone (PTH) |
| Cortisol |
| 17-
Ketosteroids (Urine) & 17-Ketogenic Steroids (Urine) |
| Vanillyl
Mandelic Acid (Urine) |
| Corticotropin
(ACTH) |
| Alpha
Feto Protein (AFP) |
| Carcino
Embryonic Antigen (CEA) |
| Prostate
Specific Antigen (PSA) |
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Cytology
is that branch of diagnostic medicine that deals
with study of structural characteristics of individual
cells spread on laboratory slide and stained appropriately.
The material is obtained by scraping the tissue
suspected to be harboring the disease, natural
secretions from those organs or by aspirating
through a fine needle. |
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Cytological examination
of the sputum is of value in the diagnosis of
malignant diseases of the lower respiratory tract,
pulmonary asbestosis & pulmonary fungal infections. |
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Gynecological cytology
has three important applications: 1)The detection
of malignant lesions |
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2)
The assessment of hormone function |
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3) The identification
of vaginal infections |
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Fine Needle Aspiration
Cytology (FNAC) has widened the scope of cytological
examinations. Any swelling, tumorous or inflammatory
in nature, superficial and easily accessible can
be aspirated with ease. The aspirated material
can be examined to diagnose both malignant and
benign (mild) conditions. |
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Histology is study
of anatomy at cellular level using a microscope.
When same tool is applied to study the abnormalities
in tissue anatomy under disease conditions to
make the diagnosis of disease, it is called histopathology.
Materials used for histopathology are tissue bits
obtained by biopsy and tissues/organs removed
during surgery. |
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CYTOLOGY |
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Sputum
for Cytology |
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| Female
Genital Tract Cytology -Vaginal/Cervical smear (PAP) |
| FNAC |
| Histopathology
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Clinical pathology refers to
a group of laboratory investigations, which are
supposed to be done in side room, adjacent to
hospital wards so as to aid in quick diagnosis
of disease and consequently to start treatment
at the earliest. |
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Analysis of urine is one of the
frequently requested test owing to the easy availability
of the material and the large body of information
gathered from it, which will provide a pointer
towards the disease process and help in ordering
further laboratory tests. Generally urine analysis
is used as a preliminary screening test rather
than using it as the definitive test to diagnose
the disease (there are some exceptions to this
statement). Analysis of urine gives information
on 1) diseases of kidney, ureter, bladder and
urethra 2) general and systemic diseases involving
whole body or some organs. By applying some special
techniques, inherited diseases of metabolism can
be diagnosed. |
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This is a screening test for
multiple myeloma. Positive test strongly suggests
the presence of disease. But negative test does
not rule out the disease. |
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A routine examination of the
stool provides diagnostic information about gut
infections, obstructive jaundice, gastrointestinal
tract bleeding and lactose intolerance. |
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Semen
examination is an integral part of the evaluation
of infertility. As a result of its relative simplicity
it is often requested before more complicated
& expensive evaluation of the female |
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partner is undertaken. The test gives qualitative
and quantitative information about seminal fluid
from which one can know the exact nature of problem.
Post coital test is performed to |
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evaluate the behavior
of sperms once it enters the female genital tract.
This test gives information about the adverse
conditions in vagina and cervix that could hamper
the sperm motility. |
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Whenever
there is clinical evidence of central nervous
system disease, examination of CSF is indicated.
CSF serves as a medium for the exchange between
the blood and the brain and |
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hence its analysis gives information about the
possible nature of pathological process in the
central nervous system. Routine examination of
CSF includes - pressure, physical examination |
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(appearance, color
& clot ) chemical examination, cytological
examination, bacteriological examination and serological
examination. |
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CLINICAL
PATHOLOGY |
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Urine
Analysis |
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| Urine
for Bence Jones |
| Stool
Routine |
| Semen
Analysis & Post Coital Test |
| Cerebrospinal
Fluid Analysis (CSF) |
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Haematology is mainly
concerned with the study of cellular formed elements
of blood, their number, the relative distribution
of various types of cells and the structural or
biochemical abnormalities that occur in blood
and blood forming organs and other systemic disorders. |
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Complete Blood count
along with Red blood cell indices and ESR. |
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Complete Blood Count
includes total count, differential count, red
blood cell count, haemoglobin, haematocrit and
platelet count. |
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Total count estimates
the total number of white cells in a cubic millimeter
of blood. It is important in the diagnosis of
disease especially when accompanied by a differential
white cell count. The information obtained from
this test will help the doctor to differentiate
between acute and chronic infections, bacterial
v/s viral infections and to diagnose cancer of
white blood cells |
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The rate of sedimentation
of RBCs estimated under standard conditions is
known as the Erythrocyte |
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Sedimentation Rate. It indicates the possible
presence of organic disease or to follow the course
of disease. Variations in ESR are of significance
only in association with other |
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indicators
significance only in association with other indicators
of infection. |
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Platelet count indicates
the no of platelets in circulating blood. Drop
in platelet count gives rise to bleeding into
the skin and internal organs. Platelet count drops
in wide range of conditions involving bone marrow
and spleen, tissue injury, cancer, infection,
alcoholic liver disease, HIV and cancer chemotherapy.
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0Abnormal increase
in the number of eosinophils in the blood is a
characteristic of allergic states & various parasitic
infections. |
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These are immature
red cells. Their number increases when there is
reduction in number of red cells in the peripheral
blood ex by acute loss of blood or abnormal breakdown
of RBCs. The RC is of value in megaloblastic Anemia's,
for improvement is indicated by a rise in reticulocytes
in the peripheral blood. |
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Examination of a
stained film of blood under microscope reveals
lot of information about various disease processes
occurring in the body as well as the diseases
affecting the blood cells themselves. It also
reveals the presence of blood borne parasitic
infections such as malaria and filariasis. |
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Blood film is examined
for LE cells in a collagen vascular disease called
systemic lupus erythematosus. This affects women
most commonly and is characterized by a skin rash,
arthralgia, fever, renal, cardiac & vascular lesions,
anemia etc. |
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One of the classes
into which individuals or their blood can be separated
on the basis of the presence or absence of specific
antigens in the blood The 4 blood groups are determined
by the presence or absence on the RBCs of the
blood groups, antigens A &B, therefore the blood
group of the individual may be
1 Group A - presence of A antigen
2 Group B - presence of B antigen
3 Group AB - presence of both A & B antigen
4 Group O - absence of both A & B antigen
Rh typing is done to detect an D antigen which
was first detected on red cells of rhesus monkey
(hence the name Rh). Its presence is taken as
Rh positive and absence as Rh negative. |
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This is screening
test performed to detect abnormal haemoglobin
known as HbS. When oxygen is liberated from HbS,
it converts the red cells to sickle shaped cell
instead of round shape. This phenomenon is studied
on a slide under microscope. |
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Haemoglobin carries
out the important function of transportation of
oxygen from lungs to various parts of the body.
Hb estimation is the first test ordered in patients
suspected to be having anaemia. Hb level is increased
in polycythemia. |
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Bleeding time and
clotting time are two screening tests performed
to evaluate the clotting mechanism of blood. This
test is very important before any invasive procedure
and also while |
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evaluating a patient with bleeding tendency. BT
is prolonged when the platelet count is decreased
or when theplatelets are qualitatively abnormal. |
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Prolonged
CT time will be noted in deficiency of clotting
factors, for eg. haemophilia. Normal clotting
time is 4 to 10 minutes. |
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Prothrombin time
is the time required for the clotting of oxalate
plasma in the presence of a standard preparation
of tissue thromboplastin. The patients value is
compared with the PT value of pooled plasma obtained
from normal people and reported as a ratio. PT
screens for the defects of extrinsic pathway of
blood coagulation(coagulation factors VII, X,
V & II). Prolonged PT is seen in patients on anticoagulant
therapy and liver disorders. The normal PT time
is about 12 to14 seconds. |
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Clot Retraction is
directly related to platelet count. In this method
whole blood is allowed to clot spontaneously.
Normally retraction of the clot from the walls
of the tube starts in 30 minutes, 50% complete
at the end of hour & complete in 24 hours. In
abnormal conditions there is no retraction even
after 24 hours. Clot Retraction is delayed in
cases of patients having platelet disorders, thrombocytopenia,
fibrinogenopenia & hypoprothrombinaemia. |
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Certain thromboplastins
lack the ability to compensate for the plasma
defect of hemophilia. These thromboplastins are
called partial thromboplastins & when such material
is used in one stage clotting tests with addition
of excess calcium the procedure is called the
activated partial thromboplastin time (aPTT or
PTT). PTT detects the defects in intrinsic pathway
of coagulation (coagulation factors XII, XI, IX,
VIII, X, V & II). PT and PTT together detect 95%
of all coagulation abnormalities. |
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The red cells, white
cells & platelets are manufactured in the bone
marrow. If the manufacturing process is abnormal
it may indicate disease of bone marrow. Bone marrow
examination is of particular interest to the physician
in the diseases such as aplastic anemia, pernicious
anemia, leukemia, purpura haemorrhagica, agranulocytosis,
lymphoma, iron overload & multiple myeloma. |
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HAEMATOLOGY |
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Haemogram |
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| Complete
Blood Count (CBC) |
| TC
& DC |
| Erythrocyte
Sedimentation Rate (ESR) |
| Platelet
Count |
| Absolute
Eosinophil Count (AEC) |
| Reticulocyte
Count |
| Blood
Smear Exam |
| Lupus
Erythematosus (LE) Cells |
| Blood
Grouping & Rh Typing |
| Sickle
Cell Test |
| Haemoglobin
Estimation |
| Bleeding
Time & Clotting Time (BT & CT) |
| Prothrombin
Time |
| Clot
Retraction (CRT) |
| Partial
Thromboplastin Time |
| Bone
Marrow Examination |
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The study of microbes
is very important because diseases caused by microbes
are potentially curable and preventable. Microbiology
has very close link with curative medicine in
regard to the precise diagnosis & the rational
treatment of diseases. |
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Culture is isolation
of bacteria causing infection on an artificial
medium and sensitivity is detecting the antibiotic
to which the isolated bacteria is most sensitive.
Materials used are - PUS, BLOOD, URINE, FAECES,
SPUTUM and BODY FLUIDS. Cultural examination of
the urine is done for the diagnosis of enteric
fevers, as also, for the detection of urinary
carriers of these infections.Bacterimia is an
important part of any systemic infection & hence
blood culture acquires significance. Blood culture
permits the prompt commencement of specific treatment
against the offending organism & may prove to
be life saving. Bacterimia occurs in pneumococcal
pneumonia, Bacterial meningitis, Urinary tract
infections, enteric fever etc. |
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It is one of the
most frequently used stains. Bacterial identification
is aided by their staining reactions. Gram staining
is done to show the presence of organisms & the
nature of the cellular contents |
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It is an abnormal
structure or foreign cell within a cell/ an intracellular
body that is characteristic of some virus diseases
& is the site of virus multiplication. |
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This method is used
to observe the morphology (form and structure
of an organism or any of its parts) but also demonstrates
the motility of organisms. |
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MICROBIOLOGY |
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Culture
& Sensitivity (C&S) |
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| Gram
Staining |
| Inclusion
Bodies |
| Hanging
Drop Preparation |
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Serology is that
branch of science dealing with serum & especially
their reactions & properties. Immunology is that
branch of science that deals with the immune system
& immune responses. |
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This test is done
to detect, identify and quantitate specific antibody
in serum samples from patients suffering from
(suspected) enteric fever or pyrexia of unknown
origin. |
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Brucella is a nonmotile
bacteria that cause disease in humans. This test
is aimed at detecting the antibody to brucella. |
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This test is done
to detect the presence of antibodies to Epstein-Barr
virus in serum |
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CRP is a plasma protein
only to be found in low concentrations in the
serum of healthy subjects. The detection of CRP
is more sensitive & more reliable indicator for
inflammatory diseases & in malignant tumors. |
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The detection of
rheumatoid factors is of high diagnostic value
in the diagnostic value in the diagnosis of rheumatoid
arthritis arrived at on the basis of case history,
clinical findings & possibly X-Ray. |
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VDRL test has been
the most popular screening test for the diagnosis
of syphilis. Syphilis is a sexually transmitted
disease caused by direct, but sometimes by indirect
contact. The causative organism Treponema pallidum
has the power of gaining entrance to the body
through minute lesions of the skin or mucus membrane.
VDRL is non-specific test and reactive samples
have to be subjected to specific tests for syphilis
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This is a specific
test for syphilis. Commonly employed procedure
is Treponema Pallidum Heamagglutination test (TPHA) |
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Toxoplasmosis is
a common infectious disease in human. This test
provides quantitative measurement of human anti
taxo IgG and IgM. Transmission to humans happens
mainly by eating raw or under cooked contaminated
meat or by close contact with contaminated material,
specially cat litter. |
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Rubella is a herpes
virus. Infection with rubella in children and
adult is a disease characterized by an erythematous
rash, mild upper respiratory symptoms and lymphadenopathy.
This test provides a reliable method for the diagnosis
of acute rubella infections screening for rubella
immunity, diagnosis for congenital infections
and vaccination follow up. |
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It is a herpes virus,
which usually causes sub clinical or mild disorders
in humans. CMV can also cause conginental abnormalities
including mental deficiency and deafness in infants
infected in utero. |
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It is an inflammatory
virus disease of the skin characterized by groups
of watery blisters on the skin or mucous membranes
above the waist & in the other by such blisters
on the genitals. |
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It is a test which
will detect antibodies to four organisms namely
toxoplasma, rubella, CMV, and Herpes simplex virus.
Infection with these viruses can cause congenital
anomalies. |
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It is test using
an antiglobulin serum to detect the antibody coating
the surface of red cells against D antigen or
Rh factor. Coomb's test direct is done to identify
hemolytic diseases of the newborn, autoimmune
hemolytic anemia, to identify incompatible blood
transfusion and drug induced hemolytic anemia.
Coomb's indirect test is one of the most reliable
methods for cross matching. It is frequently used
for determination of certain blood factors for
duffy, kell and kidd. |
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This test is conducted
to confirm tuberculosis. |
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This test is performed
as a screening procedure for Hepatitis B virus
infection either past or present. |
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This
test is a rapid visual sensitive and accurate
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immuno
assay for the combined detection of HIV I and
II Antibodies in human serum or plasma. |
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SEROLOGY
& IMMUNOLOGY |
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Widal
Test |
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| Brucella
Agglutination Test |
| Paul
Bunnel test |
| C-Reactive
Protein |
| Rheumatoid
Factor |
| VDRL
Test |
| Treponema
pallidum Ab |
| Toxoplasma
Ab |
| Rubella
Antibody |
| Cytomegalovirus
(CMV) |
| Herpes
Simplex |
| Total
TORCH Panel |
| Coomb's
Test- Direct & indirect |
| Mantoux
Test |
| Hepatitis
B Surface Antigen |
| Human
Immuno Deficiency Virus [HIV] I & II |
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